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nuclease-free
"Activation of UTP-sensitive P2Y2 receptor induces the expression of cholinergic genes in cultured cortical neurons: a signaling cascade triggered by Ca2+ mobilization and extracellular regulated kinase phosphorylation
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modified uridine nucleotide supplied for enzymatic incorporation into RNA during IVT using RNA polymerases such as T7 RNA polymerase
and Drew Weissman
DMT-2'-F-dG(iBu)-CE Phosphoramidite Application_Other Raw material nuclease-freeDMT 2 F dG(iBu) CE Phosphoramidite is a 2' fluoro (2' F) phosphoramidite monomer used for solid phase oligonucleotide synthesis. It contains a 2' F modification on the sugar of deoxyguanosine (dG) with isobutyryl (iBu) base protection, a 5 dimethoxytrityl (DMT) group for 5' hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite. Synthesis of RNA oligonucleotides using 2' F phosphoramidite increases the nuclease resistance and binding
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