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the substrate TMB is added to develop color
The phosphorylation at these sites
TMB is converted to blue under the catalysis of peroxidase (HRP) and to the final yellow under the action of acid
and axonin-1
recognizing and cutting Glu carboxy-terminal peptide bonds in NH4HCO3 of pH7
APC Mouse IgM, κ Isotype Control Antibody (S-844-28) Size:100μl the substrate TMB is addedProduct Specification Host Mouse Immunogen Recombinant Protein Clone Number S 844 28 Antibody Type Mouse mAb Isotype IgM,k Application FCM, ICFCM Concentration 0. 2 mg ml Conjugation APC Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied Background Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations
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