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His Tag Physical Appearance Liquid Storage Buffer 50 mM Tris
and HRP conjugate are sequentially added to microwells pre-coated with an uncarboxylated osteocalcin (ucOC) capture antibody
Add the Stop Solution in the same order as for adding the TMB substrate
Reference [1] Nicholson AW
Tissue homogenate: Take an appropriate amount of tissue block
Human CYBb ELISA Kit Size:96T His Tag Physical Appearance LiquidProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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